Normally, the infections in humans are asymptomatic, but at times the parasite can have serious consequences. Host animals infected with toxoplasmosis may appear to be fever, lymphadenopathy, ocular inflammation and central nervous system disturbances etc. The infection of toxoplasmosis in different animals can have various clinical symptoms. gondii include ingestion of water or food contaminated with oocysts shed by felids, ingestion of meat containing active tissue bradyzoites, and vertical transmission during gestation 4. In humans or other intermediate hosts, the routes of infection with T. One cat may shed millions of oocysts within a week during primary infection 2, 3. As a common courtyard animal, domestic cat is a considerable risk factor for the infection of T. Felids are the only known definitive hosts, which play a key role in the spread of diseases by excreting oocysts of T. gondii) is an obligate intracellular pathogen which can cause infection in almost all warm-blooded animals, including humans 1. gondii infection in this region, and contribute to a prevention and treatment program for toxoplasmosis in Jilin, China. Data analysis indicated that the positive rate in captive farm (3.2%, 95% CI: 0.0–6.7%) was significantly lower than those in cage-free farm (P < 0.05), and the region of Shulan was protective factor (OR: 0.063, 95% CI: 0.007–0.559).The results of our study alert people to be aware that the present of equids T. Statistical analysis revealed that farming model and region may be two main risk factors. The highest positive rate was observed in equids from Dongfeng (16.3%) followed by Taonan (10.0%), Wangqing (8.3%), Antu (8.0%), Tonghua (8.0%) and Shulan (2.3%). gondii molecular occurrence was 9.0% (22/245). gondii B1 gene was analyzed using multivariable logistic regression to evaluate risk factors associated with the positive rates in equids. In this study, a total of 245 blood samples of equids (192 horses, 25 donkeys and 28 mules) were collected from six localities in Jilin Province from March 2018 to August 2020 and detected by PCR. Therefore, the present study aimed to evaluate the occurrence and risk factors of Toxoplasma gondii infections in equids from Jilin, northeastern China. As the intermediate hosts, little is known about equids toxoplasmosis in Jilin province. The products of equids, such as meat and milk, have been closely related to humans’ life. Toxoplasma gondii, one of the important zoonotic parasites, has been detected in lots of hosts including humans, with a widespread prevalence.